Ameriflight is an American cargo airline based in Burbank, California, USA. It is the largest United States FAA Part 135 cargo carrier, operating scheduled and contract cargo services to destinations in 30 US states, Canada, Mexico, and the Caribbean. Ameriflight serves major financial institutions, freight forwarders, laboratories and overnight couriers in the western USA and provides feeder services for overnight express carriers nationwide.

The majority of Ameriflight's flying consists of "air feeder" service for major package express integrators such as UPS and FedEx. On schedules set by the customers, cargo is received in the early morning from large jet freighters at hub airports and distributed by Ameriflight airplanes to smaller communities whose traffic (or airports) wouldn't support the big airplanes. In the evening, the Ameriflight aircraft fly inbound to the hubs with cargo to be transferred to the big jet freighters, which carry it onward to the integrators' distribution centers for sorting and redistribution to the ultimate destinations.

Although demand is decreasing as use of digital imaging and electronic data transfer increases, the company also transports high priority intercity financial documents. Pharmaceuticals, film for development, medical laboratory samples, and other miscellaneous cargo are also carried.

The Cincinnati-Northern Kentucky International Airport (CVG) base is unique to Ameriflight as a large part of their business is last-minute on-demand auto parts and other time critical parts needed to keep assembly lines moving. CVG has also been growing Ameriflight's time-critical radioactive medical raw materials business.

The Dallas-Fort Worth International Airport (DFW) base is unique in that it has both scheduled/contracted flights and on-demand cargo charter service. With spare Metroliner aircraft and crew based at DFW, the base chiefly serves requests for transportation originating from Texas customers destined for all parts of US, and to Tijuana International Airport and Hermosillo International Airport in Mexico. The DFW base also ships time-critical radioactive medical material.

In addition, the company conducts on-demand passenger charter operations using three Learjet 35As and a Beechcraft King Air 200.